By Mašán, Peter, Fenďa, Peter
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Additional resources for A review of the laelapid mites associated with terrestrial mammals in Slovakia, with a key to the European species. - 1
75 with HC1, while rapidly stirring at room temperature. 75. 75. 2. 5, and the modified PEI is dialyzed using 3500 molecular weight cutoff membranes against Milli Q water for 48 hr with 2 changes of water per day at 4 ° . The amount of sugar (as galactose) conjugated with PEI is determined by the phenol/sulfuric acid 41 or resorcinol method. 42 We have found that the resorcinol method is easier and more reproducible than the phenol/sulfuric acid determination. ) 6 mg/ml in Milli Q water is made up every 30 days and stored at 4 ° in the dark.
Wu, and G. Y. 247, 341 (1994). [9,] SITE-DIRECTED GENE REPAIR 29 FIG. 5. HUH-7 cell uptake of fluorescein-labeled ONs. Cells were incubated with 1/zg of labeled ONs either complexed with L-PEI (top) or naked (bottom). Confocal microscopy shows significant uptake and nuclear localization of the fluorescently-labeled ONs only with L-PEI (top). Uptake by the ASGPR is significantly inhibited by 100 mM D-galactose (middle). ON, Oligonucleotide; Gal, galactose. 30 NONVIRAL  Detection and Characterization of the Site-Directed Genomic DNA M o d i f i c a t i o n DNA Isolation and Subcloning The cultured cells transfected with the ONs and control cultures are harvested 48 hr following transfection.
Process 23 that were not removed by density centrifugation do not exhibit the electron dense internal structure (see arrows in Fig. 5a). Nor is this internal structure observed in the LUV produced by extrusion (Fig. 5b). It may also be noted that SPLP visualized by cryoelectron microscopy have a remarkably homogeneous size (diameter 72 4- 5 nm), in close agreement with measurements of SPLP employing 23 L. T. Mimms, G. Zampighi, Y. Nozaki, C. Tanford, and J. A. Reynolds, Biochemistry 20, 833 (1981).