Advanced Optical Flow Cytometry: Methods and Disease by Valery V. Tuchin

By Valery V. Tuchin

An in depth examine the most recent learn in non-invasive in vivo cytometry and its purposes, with specific emphasis on novel biophotonic tools, illness analysis, and tracking of affliction remedy at unmarried mobile point in desk bound and circulate stipulations.
This booklet therefore covers the spectrum starting from primary interactions among gentle, cells, vascular tissue, and telephone labeling debris, to concepts and possibilities for preclinical and scientific study. common themes contain mild scattering through cells, speedy video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic equipment for mobile diagnostics and tracking of ailment therapy in dwelling organisms. additionally offered are discussions of complex equipment and methods of classical stream cytometry.

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Lymphocytes were subtyped by their CD3 and CD16 expression: CD3+ CD16− , CD3+ CD16+ , CD3− CD16− , and CD3− CD16+ . CD19 expression allowed for unequivocal identification of B cells in the CD3− CD16− population. ) on a single cell level, expression of every marker can be verified for each cell, or more practically, for each identified cell population. Without further investigation, one would expect all the CD3+ CD16− population to be T cells. But that is not the truth. CD56 expression revealed a small population (∼7%) of CD3+ CD16− NKT cells.

Leukocytes were further subdivided by their different antigen expression. Figure was published earlier in: Mittag et al. [22]. 2 Polychromatic characterization of leukocytes. Detailed subtyping of cells, leukocytes in this example, is only possible with polychromatic cytometry, that is, the simultaneous use of several markers. Figure was published earlier in: Mittag [48]. to find interactions and causal connections. Thus, there is an increasing demand for multiparametric analyses in the field of biological and clinical investigations.

Some promising efforts were made for label-free analysis of cells. Impedance analysis is one of them. With this technology it is possible to analyze adherent cells, for example, for detection of ischemic effects on cardiomyocytes [72] or detection of tau hyperphosphorylation in a neuroblastoma cell line [73]. Impedance analysis is also feasible with flow cytometric techniques, that is, analysis of cells in flow [74]. Cells can be analyzed at different frequencies. 1–1 MHz), membrane capacitance (1–5 MHz), and cytoplasm conductivity (3–20 MHz).

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