Applications of Chimeric Genes and Hybrid Proteins, Part C: by Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N.

By Melvin I. Simon, Jeremy Thorner, Scott D. Emr, John N. Abelson

The seriously acclaimed laboratory ordinary for greater than 40 years, Methods in Enzymology is without doubt one of the so much hugely revered courses within the box of biochemistry. when you consider that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless suitable today--truly a vital e-book for researchers in all fields of lifestyles sciences.

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Extra resources for Applications of Chimeric Genes and Hybrid Proteins, Part C: Protein-Protein Interactions and Genomics, Volume 328 (Methods in Enzymology)

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A. 91, 12980 (1994); (4) R. S. Sikorski and P. Hieter, Genetics 122, 19 (1989); (5) E. A. , in "Current Protocols in Molecular Biology" (F. M. ), Vol. 1. John Wiley & Sons, New York, 1999; (6) J. Gyuris, E. Golemis, H. Chertkov, and R. Brent, Cell 75, 791 (1993); (7) C. W. Xu, A. R. Mendelsohn, and R. Brent, Proc. Natl. Acad. Sci. A. 94, 12473 (1997). b Expression cassette indicates the promoter (from the ADH1 or G A L l gene, or a minimal promoter with eight LexA-binding sites), followed by the fusion moiety (LexA DBD or B42 AD), or reporter (lacZ).

F. Cheng, and H. Weintraub, MoL Cell. BioL 15, 3813 (1995). [2] LexA-BASED TWO-HYBRID SYSTEMS 19 not appreciably activate transcription of the lexA operator-based reporter genes. Results from these control experiments will dictate if the Bait can be used to screen a library, using the initial test conditions, or if modifications of the Bait and/or different combinations of reporter strains/plasmids must be used. Methods. The following protocols utilize basic yeast media and transformation procedures, described in greater detail in Refs.

T h e p r o t o c o l s p r e s e n t e d h e r e w e r e d e v e l o p e d w i t h t h e y e a s t strains a n d p l a s m i d s i n d i c a t e d in T a b l e I. R e c i p e s for 8 j. Gyuris, E. Golemis, H. Chertkov, and R. Brent, Cell 75, 791 (1993). 9 R. L. Finley, Jr. and R. Brent, in "DNA Cloning, Expression Systems: A Practical Approach" (B. D. Haines and D. M. ), pp. 169-203. Oxford University Press, Oxford, 1995. [3] I N T E R A C T I O N M A T I N G T W O - H Y B R I D SYSTEMS 29 yeast media can be found elsewhere in this volume 1° and are available at our Web site.

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